human ec cell lines  (ATCC)

Journal: Scientific Reports

Article Title: Integrative network toxicology and experimental evidence reveal mechanisms underlying diethyl phthalate-induced initiation and progression of endometrial cancer

doi: 10.1038/s41598-026-39325-6

Figure Lengend Snippet: DEP inhibited proliferation and induced ROS accumulation in EC cells. ( A , B ) CCK-8 assay showing the effect of different concentrations of DEP on proliferation of HEC-1-A ( A ) and Ishikawa ( B ) cells at 0, 24, and 48 h (n = 3). ( C , D ) Representative EdU staining images of HEC-1-A ( C ) and Ishikawa ( D ) cells treated with DEP at the indicated concentrations (DMSO, DEP at 10 nM, 1 μM, and 10 μM). Nuclei were counterstained with Hoechst 33,342 (blue), and EdU-positive cells are shown in red. Scale bar = 100 μm. ( E ) Quantification of EdU-positive cells in HEC-1-A and Ishikawa cell lines (n = 3). ( F ) Representative fluorescence images of ROS staining in HEC-1-A and Ishikawa cells following DEP treatment. White arrows indicate cells with increased ROS levels, and blue arrows indicate cells with lower ROS levels (n = 3). Scale bar = 20 μm. Data are presented as mean ± SD; statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. (* P < 0.05, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human EC cell lines HEC-1-A and Ishikawa were obtained from GeneChem Gene Technology Co., Ltd. (Shanghai, China).

Techniques: CCK-8 Assay, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Integrative network toxicology and experimental evidence reveal mechanisms underlying diethyl phthalate-induced initiation and progression of endometrial cancer

doi: 10.1038/s41598-026-39325-6

Figure Lengend Snippet: DEP induced cell cycle arrest and modulated the PI3K/AKT signaling pathway in EC cells. ( A ) Flow cytometry analysis of cell cycle distribution in Ishikawa and HEC-1-A cells treated with DEP at the indicated concentrations (DMSO, DEP at 10 nM, 1 μM, and 10 μM). ( B ) Quantification of the proportion of cells in G0/G1, S, and G2/M phases (n = 3). ( C ) WB analysis of PI3K, P-PI3K, AKT, P-AKT, ERK, P-ERK, CDK4, Cyclin D1, GSK3β, and P-GSK3β expression in Ishikawa and HEC-1-A cells after DEP treatment. ( D ) Densitometric quantification of protein expression levels normalized to GAPDH or β-actin (n = 3). Data are presented as mean ± SD; statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test. (ns = not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human EC cell lines HEC-1-A and Ishikawa were obtained from GeneChem Gene Technology Co., Ltd. (Shanghai, China).

Techniques: Flow Cytometry, Expressing

Journal: Frontiers in Immunology

Article Title: Pan-cancer multi-omics characterization of calcyphosine and its revealed links to the immune microenvironment and regulatory networks in endometrial carcinoma

doi: 10.3389/fimmu.2025.1688606

Figure Lengend Snippet: CAPS expression in EC and its impact on cell proliferation. (A) Western blot analysis of CAPS protein levels in EC tissues and paired adjacent non-tumor tissues. (B) Quantification of CAPS protein expression based on Western blot results. (C) Representative immunohistochemical (IHC) staining images of CAPS in EC and adjacent tissues at 20× and 40× magnification. (D) Statistical analysis of IHC scores (n = 12 per group). (E, F) CCK-8 assays evaluating cell viability in KLE and Ishikawa cells following CAPS knockdown. (G, H) EdU staining assays assessing proliferative capacity after CAPS knockdown in KLE and Ishikawa cells. (I, J) Quantification of EdU-positive cells. (K) CAPS knockdown efficiency validated by Western blot in Ishikawa and KLE cells. CAPS protein levels were markedly reduced after transfection with Si1-CAPS and Si3-CAPS compared with Si-NC. β-actin served as the loading control.(* P < 0.05, ** P < 0.01, **** P < 0.0001).

Article Snippet: The human EC cell lines KLE and Ishikawa were obtained from GeneChem Co., Ltd. (Shanghai, China).

Techniques: Expressing, Western Blot, Immunohistochemical staining, Immunohistochemistry, CCK-8 Assay, Knockdown, Staining, Transfection, Control

Journal: Frontiers in Immunology

Article Title: Pan-cancer multi-omics characterization of calcyphosine and its revealed links to the immune microenvironment and regulatory networks in endometrial carcinoma

doi: 10.3389/fimmu.2025.1688606

Figure Lengend Snippet: CAPS knockdown promotes migration and invasion of EC cells. (A, B) Wound healing assays evaluating the effect of CAPS knockdown on wound closure in KLE and Ishikawa cells. (C, D) . Quantification of wound healing areas. (E, F) Transwell assays assessing the impact of CAPS knockdown on cell migration (E) and invasion (F) in KLE and Ishikawa cells. (G, H) Quantification of migrated (G) and invaded (H) cells in Transwell assays. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: The human EC cell lines KLE and Ishikawa were obtained from GeneChem Co., Ltd. (Shanghai, China).

Techniques: Knockdown, Migration